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Affinity chromatography column, HisPrep™ FF

Leverantör: GE Healthcare
HisPrep™
HisPrep™ FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow which consists of 90 mm beads of highly cross-linked agarose, to which a chelating ligand has been immobilised. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins.

  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Negligible leakage of Ni²⁺
  • High binding capacity, approximately 40 mg/ml medium
  • Convenient and time-saving 20 ml prepacked HiPrep format

Ni Sepharose™ 6 Fast Flow consists of 90 m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.




 

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Benämning Column hardware Partikelstorlek Int.Ø×L Volym Flödeshastighet Förp. VWR art.nr Antal
HisPrep™ FF 16/10 PP 90 µm 16×100 mm 20 ml <10 ml/min 1 28-9365-51
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SPECIFIKATIONER

Max. tryck 1,5 bar
pH range 3 - 12
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