3,3 ', 5,5'-tetrametylbensidin membrane peroxidase substrate Plus, liquid, Biotechnology Grade 1 component

Leverantör: VWR Chemicals

K830-100MLEA 2925 SEK
K830-100ML
3,3 ', 5,5'-tetrametylbensidin membrane peroxidase substrate Plus, liquid, Biotechnology Grade 1 component
3,3 ', 5,5'-tetrametylbensidin

Ready to use sensitive substrate for the detection of horseradish peroxidase (HRP) activity suitable for ELISA and solution assays. Offers faster signal, enhanced sensitivity and the lowest possible background.


  • Ready to use solution


Reaction of the substrate with HRP generates a free-radical cation that exhibits blue colour with an absorption maximum at 653 nm. Acidification of the reaction by addition of sulfuric acid yields a yellow-coloured diimine terminal oxidation product, which absorbs light at 450 nm.


TMB Plus liquid component substrate is optimised with TMB and hydrogen peroxide concentrations that allow a linear response to typical HRP concentrations utilised in immunologic assays.


Beställningsinformation: Contains 2,08 mmol/l TMB, hydrogen peroxide, citric acid buffer pH 3,3 and additional proprietary, non toxic stabilisers.


Förpackning: Packed in plastic bottle.

Formel: C₁₆H₂₀N₂
MW: 240,35 g/mol
MDL Number: MFCD00007748
CAS nummer: 54827-17-7

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Specifikation testresultat

Abs.@450nm (50%, 1M H2SO4) ≤ 0.1
Abs.@650nm (undiluted) ≤ 0.1
Assay (P/F) PASS / FAIL
pH @25C (undiluted) 2.6 - 3.6

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