Linear acrylamide

K548-5X1MLEA 2375 SEK
VWRCK548-5X1ML
Linear acrylamide
Linear acrylamide is a co-precipitant that improves the yield of nucleic acids in the precipitation steps of purification protocols.

  • Free from DNase and RNase

The purification of nucleic acids for PCR, RT-PCR and other enzymatic reactions by alcohol precipitation is an essential step to yield high quality results. Traditional alcohol precipitation procedures require co-precipitants like glycogen or yeast RNA to improve recovery of nucleic acids. Linear polyacrylamide has also been shown to effectively precipitate nucleic acids (down to picogram quantities) when used with ethanol. Specifically, DNA fragments > 20 base pairs have been shown to precipitate from solution while shorter fragments and free nucleotides remain in the supernatant. This easily dispensed acrylamide solution is a neutral carrier and is derived from a non-biological source. As a result, it is free from nucleic acid or nuclease contamination. Furthermore, it does not interfere with A260/A280 readings.
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