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Anti-CALR Rabbit Polyclonal Antibody

Leverantör: ProSci Inc.
Calreticulin is a multifunctional protein that acts as a major Ca (2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

CALR antibody can be used for detection of CALR by ELISA at 1:12500. CALR antibody can be used for detection of CALR by western blot at 1.0-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Antibody is purified by protein A chromatography method.

Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type:
Antigen: CALR
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human




 

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Storlek Förp. VWR art.nr Antal
100 µg 100 µG PRSI27-296
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SPECIFIKATIONER

Antigen symbol CALR
Antigen name Calreticulin
Reaktivitet Human
Conjugation Unconjugated
Clonality Polyclonal
Värd Rabbit
Western blot Yes
ELISA Yes
Antigen synonymer SSA, ERp60, cC1qR, CRT, RO, HEL-S-99n, grp60, Calregulin, Endoplasmic Reticulum Resident Protein 60, calreticulin
Immunogen A synthetic peptide corresponding a region of human CALR.
Form Lyophilized
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