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Anti-NFYA Rabbit Polyclonal Antibody

Leverantör: ProSci Inc.
NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.

NFYA antibody can be used for detection of NFYA by ELISA at 1:312500. NFYA antibody can be used for detection of NFYA by western blot at 5.0-8.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Antibody is supplied as total IgG.

Antibody is lyophilized in PBS buffer with 2% sucrose. Add 200 μL of distilled water. Final antibody concentration is 1 mg/mL.

Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Type:
Antigen: NFYA
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Mouse, Rat, Dog




 

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Storlek Förp. VWR art.nr Antal
100 µg 100 µG PRSI27-320
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SPECIFIKATIONER

Antigen symbol NFYA
Antigen name Nuclear Transcription Factor Y, Alpha
Reaktivitet Human, Rat, Dog, Mouse
Conjugation Unconjugated
Clonality Polyclonal
Värd Rabbit
Western blot Yes
ELISA Yes
ImmunoChemistry Yes
Antigen synonymer CBF-B, CBF-A, HAP2, NF-YA
Immunogen A synthetic peptide corresponding a region of human NFYA.
Form Lyophilized
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