Rapid sequencing kits

Leverantör: Oxford Nanopore Technologies
Rapid sequencing kits
Nukleinsyrareagens Next Generation Sequencing Reagents
Rapid Sequencing Kits offer a simple and rapid library preparation method for genomic DNA sequencing on MinION™ devices.

  • Sequence native DNA — eliminate PCR bias and retain base modifications
  • Fast library prep — in as little as 10 minutes from extracted DNA
  • Minimal lab equipment required
  • Multiplex samples with rapid barcoding kits

Rapid sequencing kits generate sequencing libraries from extracted gDNA in as little as 10 minutes* using a simple, streamlined, workflow — requiring minimal lab equipment. With no requirement for amplification, these kits eliminate potential PCR bias and preserve base modifications (e.g. methylation), which can be detected alongside the nucleotide sequence.

At the heart of these kits is a transposase which simultaneously cleaves template molecules and attaches tags to the cleaved ends. Rapid Sequencing Adapters are then added to the tagged ends.

Rapid Sequencing Kits are optimised for simplicity and speed, rather than for obtaining maximum throughput. Due to the simple nature of the workflow and the fact that little sample manipulation is required (e.g. minimal pipetting steps and no clean-ups), some very long reads can be achieved with these kits, despite the required transposase fragmentation. However, in order for long reads to be observed in sequencing, long DNA fragments must be present in the sample.

In addition to the singleplex Rapid Sequencing Kit, which enables one sample to be sequenced per run, two standalone rapid barcoding kits are available: the rapid barcoding kit and rapid barcoding kit 96, which enable multiplexing of up to 12 and 96 samples per run, respectively. barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together, making more efficient use of the flow cell.

Rapid barcoding kts attach barcoded tags to the cleaved ends of the template molecules. Barcoded samples are pooled, and rapid sequencing adapters are then added to the tagged ends. Deconvolution of barcoded sequencing data is supported by the MinKNOW and Guppy software provided with MinION sequencing devices, which classify the barcode sequence and sort reads into corresponding folders.

The rapid sequencing Kit and rapid barcoding kit recommend a total input of 400 ng high molecular weight (>30 kb) gDNA per sample, while the rapid barcoding kit 96 recommends 50 ng high molecular weight (>30 kb) gDNA per sample. Addition of less input DNA, or shorter fragments could compromise sequencing throughput and read length.

*Rapid sequencing kit and rapid barcoding kit. Approximately 60 minutes for rapid barcoding kit 96.

Leveransinformation: Flow cells and kits are shipped together at 2 to 8 °C. Upon receipt, please place the product in a long-term storage location at ‒20 °C. Oxford Nanopore Technologies deem the useful life of the product to be three months from receipt by the customer.

Observera: Oxford Nanopore Technologies products are not intended for use for health assessment or to diagnose, treat, mitigate, cure, or prevent any disease or condition.
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